Fig. 1.

Breeding strategy and verification of GluN2B KO mice. (A) Schematic of breeding strategy for production of GluN2B KO mice. Mice are homozygous for the GluN2Bfl/fl allele and heterozygous for either the CaMKIIα-tTA transgene or tetO-CRE transgene. (B) Western blot analysis of GluN2B, GluN1, and GluN2A expression levels in dlBNST (representative image of punch locations marked with yellow arrow) of control (single-transgene and WT littermates) and GluN2B KO mice. (C) (Left) Decay time of evoked NMDAR EPSCs in neurons from control and GluN2B KO mice. (Right) Representative traces of NMDAR EPSCs from control and GluN2B KO mice. *P < 0.05; **P < 0.01.