Skip to main content
. 2012 Jan 18;109(5):1655–1660. doi: 10.1073/pnas.1120588109

Fig. 2.

Fig. 2.

Effects of the GHRH antagonist JMR-132 on the expression of GHRH and its receptors, activation of ERK1/2, and activation of Akt in tumor samples of PC-3 human androgen-independent prostate cancer xenografted into nude mice. (A) Western blot analysis of GHRH, GHRH-R, and SV1 in PC-3 tumor samples. Representative blots of three independent experiments are presented and include β-actin as an internal standard. Representative bands for each experimental group were grouped digitally. (B) Western blot analysis of pERK1/2 and total ERK1/2. Representative blots of three independent experiments are presented. Representative bands for each experimental group were grouped digitally. (C) Relative expression of pERK1/2. Bars represent average relative intensity values of pERK1/2 compared with total ERK1/2. *P < 0.05 compared with control by Student's t test. (D) Western blot analysis of phosphorylated Akt (pAkt) and total Akt. Representative blots of three independent experiments are presented. Representative bands for each experimental group were grouped digitally. (E) Relative expression of pAkt. Bars represent average relative intensity values of pAkt compared with total Akt. Data are shown as means ± SEM. *P < 0.05 by Student's t test.