Fig. 3.

FLNA is associated with components of the RNA Pol I machinery. (A) Coimmunoprecipitation assays of endogenous FLNA from nuclear extracts showing that FLNA is associated with actin, TIF-IA, and RPA40. (B) rRNA gene organization. ChIP assay primers for IGS (IG), promoter (P), and rRNA coding region (18S) are shown. (C–F) ChIP assays showing differential recruitment of Pol I components to the rDNA in M2 and A7 cells. Chromatin was precipitated with antibodies against TIF-IA (C), actin (D), RPA40 (E), and UBF (F). The relative enrichment was determined by qRT-PCR. Fold occupancy was calculated by dividing fold enrichment in M2 cells by that in A7 cells. The data represent three independent experiments performed in triplicate; error bars represent SEM.