Fig. 1.

Activated autologous and allogeneic CD4⁺ cells down-regulate EBNA2 and reduce LCL proliferation. LCL07.08 was cocultured with CD4⁺ T cells activated with 5 μg/mL PHA or anti-CD3/CD28 beads (A and B). At the indicated time point, LCLs were isolated from the coculture by anti-CD19 beads and analyzed by immunoblot for EBNA2 protein expression. In the experiments for C and D, CD4⁺ T cells were activated with anti-CD3/CD28 beads. They were mixed with autologous, freshly established LCLs (10 d old) and with LCL07.08 that constituted the allogeneic LCLs. On day 5, samples were handled in the same way as described in A and B. In addition, on these separated LCLs, [³H]Thy incorporation was determined.