Fig. 2.

CD4⁺ T-cell-induced down-regulation of EBNA2 and CD23 and the inhibition of proliferation do not require cell contact (TW experiments). LCL07.08 was cocultured with anti-CD3/CD28 beads activated or control CD4⁺ T cells for 5 d. In parallel, the same LCL was cultured in the TW system. On day 5, LCLs were isolated from the coculture with anti-CD19 beads and their EBV protein expression was determined by immunoblot along with LCLs from the TW (A). Control TW experiments were done with CD8⁺ T cells or with different LCLs numbers placed on the other side of the insert (B). Numbers represent the initial plating nr. of LCLs in the well surrounding the insert. [³H]Thy incorporation on day 5 of the LCLs from the insert (C). CD23, CD54, and CD95 expression was determined by FACS staining on LCLs cultured in TW (D).