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Changes at mRNA level after the LCL-CD4+ T-cell soluble factor encounter. LCL05.18 was subjected to activated CD4+ T-cell effects in TW system. On day 5, LCLs were collected and quantitative PCR was performed on them to determine their relative Cp activity (A), relative EBNA2 mRNA (B), and LMP1 mRNA levels (C). The relative mRNA levels transcribed from the terminal repeat promoter (TRp) of LMP1 was also determined and expressed as % of total LMP1 mRNA (D).
