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Kinetics of EBNA2 down-regulation. LCL07.08 was cultured in the TW system with anti-CD3/CD28 bead-activated CD4+ T cells or control CD4+ T cells. LCLs were collected at different time points, and EBV protein levels were determined by immunoblot (A). Cells recovered on day 3 were replated in fresh medium (day 0 in B). On days 4 and 10, pellets were made to assess the kinetics of EBV protein recovery after the initial 3 d of activated CD4+ T-cell exposure (B).
