Fig. 6.

Neutralization of IL21 in the activated CD4⁺ T-cell supernatants does not inhibit down-regulation of EBNA2 and [³H]Thy incorporation. LCL07.08 was treated with 50 ng/mL IL21 for 3 d. To parallel, IL21-treated cultures, 10 μg/mL anti-IL21 antibody was added. The same neutralizing anti-IL21 Ab was added to anti-CD3/CD28-activated CD4⁺ T-cell cultures that were in TW with the LCL07.08. On day 3, cells were collected and processed for EBV protein detection (A) and [³H]Thy incorporation (B). IL21 content of anti-CD3/CD28 bead-activated CD4⁺ T-cell supernatant (20 μL/lane) was determined by immunoblot (C). Recombinant IL21 of a known amount was also loaded on the gel. The smaller image above represents the film exposed to the blot for a longer time.