Fig. 7.

Soluble CD40L is partially responsible for down-regulation of EBNA2. LCL07.08 was subjected to the activated CD4⁺ T-cell effects in the TW system. To parallel cultures, anti-CD40 antibody was added. As a control, anti-CD40 antibody was added to LCLs cultured alone. On day 3, cells were collected and EBV proteins were detected. The lower band on the LMP1 blot represents the Ig heavy chain of the anti-CD40 antibody, as proven by the last lane on the immunoblot where pure anti-CD40 antibody was loaded.