Fig. 8.

EBV-infected PBMCs secrete soluble factors that down-regulate EBNA2 in freshly infected B cells. The experiment was performed in TW system. In the well, 5 × 10⁶ EBV-infected PBMCs or noninfected PBMCs were cultured (A and B). In C, the well contained 5 × 10⁶ EBV-infected PBMCs or CD8⁺ T-cell-depleted PBMCs infected with EBV. EBV-infected B cells (10⁶) were placed in the insert. On day 8 (B) or 14 (C), cells from the insert were collected and tested for their EBNA2 and LMP1 protein expression. Cell number in the insert was recorded on day 8 (A, Left), and their [³H]Thy incorporation was determined during an overnight pulse.