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. 2012 Jan 17;109(5):1661–1666. doi: 10.1073/pnas.1113166109

Fig. 4.

Fig. 4.

Sacsin loss of function alters the distribution of mitochondria in neurons. (A) Western blots (using antibodies against the indicated proteins) of lysates from cultured hippocampal neurons transduced with lentivirus encoding a control shRNAmiR (Q) or one of two different shRNAmiRs targeting sacsin (6034, 14181). The molecular mass of the proteins is indicated on the left. (B) Immunofluorescence panels of cultured hippocampal neurons (DIV14) double-labeled with chicken polyclonal antibody against MAP2 (blue) and mouse monoclonal antibody against cytochrome C (red). At DIV4, the neurons were transduced with lentivirus encoding a control shRNAmiR (Q) or one of two different shRNAmiRs targeting sacsin (6034, 14181). Arrows indicate mitochondria accumulating in proximal dendrites. (Scale bar: 40 μm.)