Table 3.
Monoclonal Antibodies Directed against Ml. Antigenic Components and Cell Markers
| MAb | Isotype | Specificity | Working dilution | Reference/source |
|---|---|---|---|---|
| Ml. antigenic components | ||||
| F126-5 | IgG1 | 36-kd protein | 1:1000 | 33 |
| F67-2 | IgG1 | 65-kd protein | 1:500 | 34 |
| F30-5 | IgM | LAM | 1:5000 | 35 |
| DZ-1 | IgG1 | PGL-I | 1:1000 | 36 |
| Cell markers | ||||
| Leu4 | IgG1 | CD3, pan T cell | 1:10 | Becton Dickinson, Mountain View, CA |
| Leu4 FITC | IgG1 | CD3, pan T cell | 1:200 | Becton Dickinson |
| EBM-11 | IgG1 | CD68, macrophage | 1:50 | Dakopatts, Glostrup, Denmark |
| EBM-11-Bio | IgG1 | CD68, macrophage | 1:100 | Becton Dickinson* |
| OKT6 | IgG1 | CD1a, Langerhans cell | 1:25 | Ortho Diagnostics, Raritan, NJ |
| OKT6-FITC | IgG1 | CD1a, Langerhans cell | 1:100 | Ortho Diagnostics |
*The MAb was biotinylated in our laboratory.