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. 2011 Nov 1;20(2):382–390. doi: 10.1038/mt.2011.236

Figure 2.

Figure 2

In vitro and in vivo potency of chemically modified small interfering RNAs (siRNAs) against tumor necrosis factor (TNFα). (a) Amount of TNFα produced after 24 hours of transfection of 50 nmol/l of siRNAs in HeLa cells expressing mouse TNFα from an exogenous expression cassette. siTNF, unmodified siRNA against TNF-α; siTNF-OMe-P, siRNA anti-TNF-α modified with 2′-O-methyl and propanediol; siControl, negative control siRNA modified with 2′-O-methyl. The data represent the mean ± SEM, n = 3. +++P < 0.001 versus siControl; **P < 0.01 versus siTNF. ANOVA, Bonferroni post-hoc test. (b) Amount of TNFα produced after 24 hours of transfection of 100 nmol/l of siRNAs in 4T1 cells. The data represent the mean ± SEM, n = 3. ++P < 0.01 versus siControl; **P < 0.01 versus siTNF. ANOVA, Bonferroni post-hoc test. (c) TNFα mRNA levels in the colon of an animal model of chronic colitis 56 hours. after rectal infusion of 4 nmol of siTNF-OMe-P (n = 4) or siControl (n = 4) conjugated with lipofectamine 2000. TNFα mRNA was measured by quantitative reverse transcriptase (qRT)-PCR and normalized with Gapdh. The data represent the mean ± SEM with respect to healthy mice (n = 9). Statistical analysis was performed using a non-parametric ANOVA with Kruskal–Wallis post-test. **P < 0.01 versus healthy mice.