Figure 3.

Antiangiogenic effect of 34.5ENVE in vitro. (a) Images of endothelial cell tube formation after being cultured on Matrigel. Endothelial cells were incubated with conditioned medium (CM) derived from U251 cells infected with the indicated virus or phosphate-buffered saline (PBS). (b) Quantification of the average number of tubes/view field (n = 4/group) observed above. (c) Inhibition of endothelial cell migration: human dermal microvascular endothelial cells (HDMECs) were incubated with CM derived from U251 cells treated with PBS, or the indicated virus. Cells were plated in the upper chamber of transwell and allowed to migrate toward CM used as a chemoattractant in the bottom chamber. The migrated cells on the bottom side of the filter were quantified as described (n = 4/group).²⁶ Data are presented as mean ± SEM of number of cells/view field.