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. 2012 Jan 30;3:9. doi: 10.3389/fphys.2012.00009

Figure 2.

Figure 2

Canine KCNJ12 genomic structure. (A) Representation of the KCNJ12 gene indicating lengths of first, second, and third exons, first and second intron, and the open reading frame (ORF). Relative position of KCNJ12 specific primers used for intron/exon mapping, 5′ and 3′ RACE are indicated by numbered box arrows 1–9. Relative positions of KCNJ12 specific primers for cloning the ORF are indicated by numbered box arrows 10–13. (B) Transcription initiation site (bold and underlined) with upstream (lower case) and downstream exon 1 (upper case) sequences. (C) Sequence surrounding exon 1–intron 1, intron 1–exon 2, exon 2–intron 2, and intron 2–exon 3 splicing sites of dog KCNJ12. Open bars indicate intron regions, hatched bar indicates exon 2 region. Exon sequences are indicated in upper case lettering, intron sequences in lower case. Consensus splicing sequences are indicated above the sites (underlined). (D) 3′ transcript terminus (bold). Exon 3 sequence is indicated in upper case, downstream genomic sequence in lower case. Consensus termination signals are indicated above the sequences (underlined).