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. 2012 Jan 30;3:9. doi: 10.3389/fphys.2012.00009

Figure 4.

Figure 4

Characterization of CfKIR2.2. (A) Western blot depicting human (Hs) and dog (Cf) KIR2.2 expression upon ectopic expression in COS-7 cells. Non-transfected cells were used as negative control. (B) Subcellular localization of ectopic human (Hs) and dog (Cf) KIR2.2 in HEK293 cells. (C) Current traces of CfKIR2.2 carried IK1, before (control), upon 100 μM BaCl2 application (Ba2+), and following washout (washout). Horizontal bar: 200 ms; Vertical bar: 2 nA. (D) Current–Voltage relationship of dog (Cf, blue squares and line; n = 8, average ± SEM) and human (Hs, red circles and line; n = 4, average ± SEM) KIR2.2 carried IK1 in HEK293T cells.