Figure 1.

The strategy to identify the human EV71 receptor, SCARB2. Mouse L929 cells became susceptible to EV71 when a human genomic DNA (red) was integrated into the genome (Ltr051 cells). RNA was prepared from Ltr051 cells and parental L929 cells and labeled with Cy3 or Cy5, respectively. The labeled RNAs were competitively hybridized to a microarray for human transcriptome analysis. The RNA species that were expressed in the Ltr051 cells but not in L929 cells were selected as candidates for the receptor mRNA. Each candidate was expressed in mouse L929 cells to examine the EV71 susceptibility of the transfected cells. SCARB2 expression alone was sufficient to achieve susceptibility.