Figure 2.

Schematic diagram of reverse genetics of wild-type MV expressing EGFP and infection of monkeys. A plasmid carrying the full-genome cDNA of the IC-B strain and the EGFP gene under the control of the T7 promoter is introduced into CHO cells expressing human SLAM (CHO/hSLAM), along with three supporting plasmids expressing N, P, and L proteins, respectively, under the control of the T7 promoter. After infection with vaccinia virus expressing the T7 RNA polymerase, wild-type MV expressing EGFP can be recovered by mixing with B95a cells. EGFP fluorescence in tissues and organs of infected monkeys can be detected using a fluorescence microscope.