Table 1.

Absence of acute and chronic cardiac allograft rejection in RAG^−/− recipients

Donor	Recipient	Recipient Treatment	Harvest Time (Days)	n	ARa	CRb
B6 RAG−/−	B6 RAG−/−	None	72 – 75	6	0	0
BALB/c RAG−/−	B6 RAG−/−	1×107 B6 T cells i.v.c	11–14d	3	3	0
B10.BR	B6 RAG−/−	None	79 – 82	7	0	0
B6 RAG−/−	BALB/c RAG−/−	CFAe	77 – 85	7	0	0
B6 RAG−/−	BALB/c RAG−/−	Poly I:Cf	60 – 75	4	0	0
B6 RAG−/−	BALB/c RAG−/−	CpGf	70 – 75	4	0	0
B6 RAG−/−	BALB/c RAG−/−	B-1 reconstitution	50 (n = 2) 90 (n = 1)	3	0	0
B6 RAG−/−	BALB/c RAG−/−	Alloimmunizationg	49 – 57
B6 RAG−/−	BALB/c RAG−/−	Alloimmunization + CFA	48 – 57	7	0	0

^aAR = Acute Rejection

^b. CR = Chronic Rejection.

^cT cells were injected 50 days after transplantation.

^dHearts were harvested upon cessation of palpable heart beat (median graft survival = 11.5 days after T cell injection) and rejection was confirmed histologically.

^eCFA (200 μl) was administered i.p. 7 days prior to transplantation.

^fPoly I:C (100 μg) and CpG-1826 (25 μg) were administered i.v. on the day of transplantation and once or twice weekly thereafter.

^gAlloimmunization was performed by injecting 2 × 10⁷ B6 RAG^−/− splenocytes i.p. 7 days prior to transplantation.