Figure 3.

Aberrant expression of Ebf3 and Shox2, but not Lmo1, at E18 in Arx^(GCG)7 mice. Wild-type and mutant E18 brain tissue were stained for Lmo1 (A–C), Shox2 (D–F) and Ebf3 (G–I) in red, with blue DAPI counterstain. The three proteins show no expression (equal to repression) in wild-type tissue (A, D, G), but are expressed (not repressed) in Arx^-/Y tissue (C, F, I). In Arx^(GCG)7/Y tissue, Lmo1 continues to be repressed (B), but Shox2 and Ebf3 are not repressed, as seen in the Arx^-/Y tissue (E and H). All images are from the ventral subpallium, with the ventricle to the left, and dorsal at the top, as indicated by the compass. Staining was performed for n = 4 for each genotype/antibody combination. Scale bar (see I): 120 mm.