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In preeclampsia, staining of invasive cytotrophoblasts with anti-VEGF-A decreased. In the third trimester of normal pregnancy (38 weeks), cytotrophoblasts (CTBs) in the interstitium (arrow) and in the walls of blood vessels (BVs) (arrowheads) stained for VEGF-A (B). In preeclampsia (PE), staining of invasive cytotrophoblasts for VEGF-A was strikingly down-regulated (D and F). CK, cytokeratin; VS, villus stroma.
