Table 2.
Dogs given 211At-anti-CD45 mAb and DLA-identical marrow grafts
| Dog ID | Weight, kg | 211At, μCi/kg | 211At, MBq/kg | Marrow TNC, × 108/kg | Donor chimerism, % (maximum to final) |
Survival, wk | Cause of death | Pathology | ||
|---|---|---|---|---|---|---|---|---|---|---|
| MNC | Granulocytes | CD3+ | ||||||||
| H349 | 8.7 | 155 | 5.9 | 2.2 | 21-5 | 34-2 | 6-4 | 18 | Low donor chimerism* | Normal† |
| H308 | 7.4 | 203 | 7.4 | 3.1 | 58-38 | 85-57 | 41-41 | 52 | End of study | Normal† |
| H232 | 8.9 | 285 | 10.7 | 5.5 | 68-42 | 100-63 | 39-35 | 53 | End of study | Normal† |
| H267 | 8.9 | 356 | 13.3 | 7.9 | 55-33 | 85-62 | 42-32 | 52 | End of study | Histologic changes in the liver‡ and thyroid§ |
| H251 | 9.6 | 411 | 15.2 | 4.9 | 60-48 | 95-59 | 69-46 | 52 | End of study | Decreased bone marrow cellularity‖ |
| H379 | 5.7 | 517 | 19.2 | 4.6 | 100-49 | 98-79 | 41-41 | 40 | End of study | Normal† |
| H388 | 6.7 | 539 | 20.0 | 7.8 | 84-58 | 100-66 | 46-46 | 49 | End of study | Normal† |
| H271 | 8.4 | 625 | 22.9 | 3.0 | 86-19 | 97-41 | 57-13 | 48 | End of study | Normal† |
Dogs were treated with 211At-labeled anti-CD45 mAb 3 days before DLA-identical bone marrow transplantation. The total dose of anti-CD45 mAb was 0.5 mg/kg, whereof 10% was injected as unlabeled anti-CD45 mAb 1 hour before treatment with the radioimmunoconjugate. Posttransplantation immunosuppression consisted of cyclosporine 15 mg/kg twice daily from day −1 to day 35 and mycophenolate mofetil 10 mg/kg twice daily from day 0 to day 27.
TNC indicates total nucleated cell.
H349 was euthanized 18 weeks after transplantation because of low-level donor chimerism.
No macroscopic or histologic evidence of radioactivity induced organ toxicity.
Histologic examination of liver tissue revealed mild nodular regenerating hyperplasia.
Thyroid hypercellularity with atrophic follicles.
Slightly decreased to 75% of a normal dog.