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. Author manuscript; available in PMC: 2012 Feb 10.
Published in final edited form as: Stem Cells. 2011 Aug;29(8):1294–1303. doi: 10.1002/stem.667

Figure 1.

Figure 1

Characterization of bone marrow stromal cells (BMSCs) derived from the rat. (A): An example of BMSC culture at 5 days after plating. Note spindle-shaped cell bodies with a round nucleus and thin processes. Scale bar = 20 µm. (B, C): Flow cytometry analysis of cultured BMSCs at 5 days after plating. Left, SSC versus FSC scatter graphs; Right, plots of the number of positive immunoreactive cells versus relative fluorescence intensities. In the examples in (B, C), 90% of cells were positive for CD90 (B) and only 0.47% of cells were positive for CD45 (C). The CD90+ and CD45− surface marker phenotype is typical of BMSCs devoid of hematopoietic contamination. Isotype-matched IgG antibodies (IgG2a in (B) and IgG2b in (C)) were used as controls. Abbreviations: #Cells, number of positive immunoreactive cells; FITC, fluorescein isothiocyanate; FSC, forward scatter; SSC, side scatter.