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. 2012 Feb 2;7:467–482. doi: 10.2147/IJN.S28249

Figure 2.

Figure 2

Dimensions of the modular nanotransporter and resulting circular structures. (A) Particle size distribution for a DTox-HMP-NLS-EGF sample (66 μM) in 10 mM sodium phosphate buffer (pH 8) containing 150 mM NaCl as measured by dynamic light scattering; the mean hydrodynamic diameter of the modular nanotransporter was 10.6 ± 0.5 nm. (B) Atomic force microscopy tapping mode height image (100 × 100 nm; z-scale, 4 nm) of egg yolk phosphatidylcholine bilayer recorded in 20 mM HEPES-MES buffer with 150 mM NaCl (pH 5.5) containing 5 nM DTox-HMP-NLS-EGF. Mean diameter of circular structures was 43.1 ± 1.2 nm (±SEM) and mean width of these structures was 20.8 ± 0.8 nm (±SEM). After correction for a widening of this width caused by final sizes of tips, the mean width of the MNT was 12.5 ± 1.9 nm (±SEM).

Note: Blue and green double arrows represent the diameter and width of the circular structure, respectively.

Abbreviations: MNT, modular nanotransporter; DTox, translocation domain of diphtheria toxin; HMP, Escherichia coli hemoglobin-like protein; NLS, nuclear localization sequence; EGF, epidermal growth factor; SEM, standard error of mean.