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. 2012 Jan 10;12:10. doi: 10.1186/1471-2407-12-10

Figure 2.

Figure 2

Morphology, proliferation and apoptosis within primary tumors formed upon injection of tumorspheres. A, B. H+E staining of 5 μm paraffin-embedded tumor sections derived from patient Sample 5 at 40× and 200×, respectively. C, E. IHC and quantitation for Ki67 were performed on 5 μm paraffin-embedded tumor sections derived from patients Samples 5-9. Ki67 positive cells and total number of cells were counted using Image J software in five randomly microphotographed images from each tumor sample and represented as the mean % positive cells with SD. D, F. TUNEL staining and quantitation (as described above) for apoptosis. MDA-MB-231 human breast cancer xenograft incubated with 4 U/ml DNase I was used as a positive control for TUNEL. Values are reported as mean +/- SD.