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. 2012 Jan 10;12:10. doi: 10.1186/1471-2407-12-10

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Copyright ©2012 Marsden et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of markers for epithelial and mesenchymal lineages in tumor samples. A-G. Representative IHC demonstrating patterns of expression of E-cadherin, fibronectin, Her2/neu, cytokeratin 8, cytokeratin 14, and aldehyde dehydrogenase 1 (ALDH1) in tumors formed upon the injection tumorspheres into the mammary fat pad of female NUDE mice. 200× magnification in all panels. H. Quantitation of positive staining and total number of cells were counted using Image J software in five randomly microphotographed images from each tumor sample and represented as the mean % positive cells with SD. I. Histological scoring of Her 2 expression within tumor samples. Values are reported as mean +/- SD.