Table 1.

Formation of primary tumor and metastasis in NUDE mice implanted with tumorspheres isolated from human breast core biopsies.

Sample	Formation in mice	Latency to palpable tumor formation	Passage in Mice	Metastasis	Metastatic Latency	Age	Diagnosis	Grade	ER/PR/Her2 Status
4	0/2	N/A	N/A	Not Determined	N/A	56 years	IDC	Grade 2	ER+/PR+/Her2-
5	5/6	74 days	Yes	Yes	254 days	44 years	IDC	Grade 3	ER+/PR+/Her2-
6	6/6	47 days	Yes	Yes	232 days	62 years	IDC with lymphovascular invasion	Grade 2	ER+/PR+/Her2+
7	5/6	72 days	Yes	Yes	214 days	77 years	IDC	Grade 2	ER-/PR-/Her2-
8	4/6	35 days	Yes	Yes	248 days	63 years	IDC	Grade 2	ER+/PR+/Her2-
9	6/6	46 days	Yes	Yes	279 days	66 years	IDC	Grade 1	ER+/PR+/Her2

Tumor formation in NUDE mice following bilateral injections into the mammary fat pad of tumorspheres that were derived from the original patient biopsy. The latency or palpable tumor formation is indicated in number of days post-injection of tumorspheres into the mammary fat pad. 'Passage in mice' indicates that the primary tumor could be serially transplanted into mice to form subsequent primary tumors following in vitro formation of tumorspheres prior to injection into the mammary fat pad. Metastasis was determined by detection of human chromosome 17 by PCR using DNA isolated from mouse organs collected from mice injected with tumorspheres into the mammary fat pad. Metastatic latency is the average number of days between the injection of tumorspheres into the mammary fat pad and the detection of metastatic lesions in all organs analyzed by H+E staining