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. 2012 Feb 10;7(2):e32029. doi: 10.1371/journal.pone.0032029

Figure 3. Neuroapoptosis decreases following CO exposure.

Figure 3

TUNEL assays were performed on coronal sections 2 hours post exposure. (a) Representative sections imaged at 10× from somatosensory neocortex (NC) and hippocampus (HC) 2 hours following 3-hour exposure to air (0 ppm CO), 5 ppm CO, or 100 ppm CO are depicted. Green TUNEL positive nuclei are visible. CA1, CA3, dentate gyrus (DG) regions of HC are labeled. Scale bars, 100 µm. (b) Quantification of total TUNEL positive nuclei from NC and HC in 3–4 non-serial coronal sections is demonstrated. Values are expressed as means plus standard deviation. N = 3–4 animals per cohort. *P<.02 vs. 0 ppm. † P<.01 vs. 0 ppm.