Fig. 2.

Deletion of TAK1 results in excessive apoptosis of DCs and loss of MDPs. (A) Caspase activity in freshly isolated WT and Map3k7^DC splenic DCs, assessed by staining with FITC-conjugated VAD-FMK. (Right) The proportion of caspase⁺ cells. (B) Measurement of apoptosis of WT and Map3k7^DC splenic DCs by TUNEL staining. (C) Flow cytometry of BrdU incorporation into splenic DCs of WT and Map3k7^DC mice after an in vivo BrdU pulse for 24 h. (Right) The percentage of proliferating splenic DCs in WT and Map3k7^DC mice. (D) Flow cytometry of MDPs (Lin^–Sca-1^–CSF1R⁺) in the BM Lin^– cells of WT and Map3k7^CreER mice after 3 d of tamoxifen treatment in vivo. (Right) The proportion of MDPs among BM Lin^– cells. (E) BM cells from WT and Map3k7^CreER mice were cultured with FLT3L in the presence of 0.5 μM 4-OHT. The expression of CSF1R and CD11c was analyzed by flow cytometry at day 4 (Left) and the percentage of CSF1R⁺ cells in CD11c^– cells was calculated (Right). Data are representative of three independent experiments. Data represent the mean ± SEM.