Fig. 2.

The UbcH7/SopA interface. (A) Stereo view of the interactions between SopA N-lobe (pink) and UbcH7 (cyan). Residues making protein-protein contacts are shown in stick model. Hydrogen bonds are indicated by yellow dashed lines. (B) Testing the interface by mutagenesis. Top, His-tagged wt SopA was incubated with untagged wt or mutant UbcH7. Bottom, wt UbcH7 were incubated with His-tagged wt and mutant SopA and immobilized with Co²⁺ beads. Proteins immobilized by Co²⁺ beads were visualized by SDS-PAGE Coomassie staining.