Skip to main content
. 2012 Jan 23;109(6):1997–2002. doi: 10.1073/pnas.1116340109

Fig. 2.

Fig. 2.

Development of a BiFC assay for detecting interactions between activators and Tra1 in vivo. (A) Schematic diagram depicting the BiFC assay. Tra1 is tagged at the C terminus with the N-terminal Venus fragment (VN), and the activator (Act) is tagged at the C terminus with the C-terminal Venus fragment (VC). (B) BiFC assay monitoring interaction between Tra1 and Gal4 in vivo, as evidenced by intense YFP signal (arrowheads) in YPG. The Tra1–Gal4 interaction occurs in the nucleus, as evidenced by colocalization (arrowheads) with the DNA stain DAPI. Tra1 was tagged at either the C terminus (Upper) or N terminus (Lower). (C) BiFC assay monitoring interaction between Tra1 and Gcn4 in vivo, as evidenced by intense YFP signal in response to amino acid starvation (−His + 3-AT media).