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Identification of Tra1 mutants that are unable to interact with Gal4. (A) BiFC assay monitoring the interaction between Gal4 and the mutant Tra1 proteins. (B) Coimmunoprecipitation assay. Spt20-HA was immunoprecipitated with an anti-HA antibody, and the immunoprecipitate analyzed for the presence of Tra1. The levels of Spt20 and Tra1 in the input extract are shown.
