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Gcn4 functionally interacts with the Tra1 GID mutants. (A) Growth of tra1-mut1 and tra1-mut8 on His-lacking media containing or lacking 3-AT. Growth of WT TRA1 and gcn4-Δ strains are shown as controls. (B) BiFC assay monitoring the interaction between Tra1-VN and Gcn4-VC in His-lacking media containing or lacking 3-AT.
