Fig. 2.
The adjuvant effect of CT is independent of typical TLR and inflammasome pathways but requires type-I IFN sensitivity. (A) B6, MyD88/TRIF DKO, and ASC KO mice received 2 × 105 naïve OT-I cells 1 d before indicated epicutaneous immunization on the ear, and expansion of the OT-I population was determined 5 d after priming. These data are pooled from three independent experiments. (B) B6 or IFNAR KO mice were adoptively transferred with naïve OT-I cells 1 d before the indicated immunization (on ear skin), and expansion of donor OT-I cells was monitored 5 d later. The data are compiled from two independent experiments and similar data were obtained in an additional similar experiment.