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. 2012 Jan 20;109(6):E290-E298. doi: 10.1073/pnas.1115725109

Fig. 3.

Fig. 3.

A single amino acid change, Y618F, specifically confers PS uptake activity to Dnf1. (A) Primary sequence alignment of TM4 from Dnf1, Dnf2, Drs2, and Atp8a1. Underlined sequences are the predicted TM4; boxes are regions differing between Dnf1 and Drs2. (B) NBD-PL uptake by Dnf1[Drs2] chimeras. Dnf1[YIS → FVT] and Dnf1 Y618F both transport PS (mean ± SEM). (C) PS/PC uptake ratio representing the relative preference of each chimera for either PC or PS independent of expression. (D) NBD-lipid uptake suggests Dnf1[TM3-4], Dnf1[TM4], and Dnf1 Y618F retain specificity for the phospholipid headgroup and glycerol backbone. Dnf1[YIS → FVT] is less selective than other PS transporting chimeras based on its activity for NBD-SM. Each reported value (B–D) is the average of at least three independent samples from at least three independent experiments (mean ± SEM).