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. 2012 Jan 20;109(6):E290-E298. doi: 10.1073/pnas.1115725109

Fig. 4.

Fig. 4.

Drs2 F511Y specifically perturbs plasma membrane PS asymmetry. (A) Growth of a drs2Δ strain expressing an empty vector, WT Drs2, Drs2 F511Y, or Drs2 F511L in the presence of papuamide B demonstrates a partial loss of PS asymmetry with Drs2 F511Y and Drs2 F511L (mean ± SEM). (B) Growth of a drs2Δ strain expressing the same constructs in the presence of duramycin indicates plasma membrane PE asymmetry is maintained by WT Drs2 and Drs2 F511Y but is perturbed with Drs2 F511L (mean ± SEM). (C) Drs2 F511Y retains an ATPase specific activity comparable to WT Drs2 while Drs2 F511L has a reduced ATPase activity (mean ± SD). (D) Drs2 F511Y fully complements cold-sensitive growth defect of a drs2Δ strain but Drs2 F511L only partially complements.