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. 2012 Jan 20;109(6):E290-E298. doi: 10.1073/pnas.1115725109

Fig. 6.

Fig. 6.

F587 substitutions specifically perturb PC uptake activity by Dnf1. (A) Primary sequence alignment of TM3-LL3-4 from Dnf1, Dnf2, Drs2, and Atp8a1. Underlined sequences are the predicted TM3 and TM4; box indicate F587 in Dnf1. (B) Growth of dnf1,2,3Δdrs2Δ expressing the Dnf1 constructs indicated on synthetic defined (SD) media, SD plus edelfosine, SD plus 5FOA. (C) NBD-PL uptake indicates a defect in PC uptake by Dnf1 F587Y and Dnf1 F587L (p < 0.05) and no defect in PE uptake (p > 0.20) (mean ± SEM).