Fig. 3.

RT-PCR analysis confirms that full-length RNA transcripts are packaged inside authentic FHV virions. RNAs from each functional class were amplified and analyzed by gel electrophoresis: (A) ′1731′ retrotransposon ORF 1; (B) 18S rRNA; (C) 7SL ncRNA; and (D) enolase mRNA. Lane 1, molecular weight standard 1 Kb plus DNA ladder (Invitrogen); lane 2, amplified from DL-1 DNA (without reverse transcription); lane 3, amplified from DL-1 RNA; lane 4, amplified from RNA encapsidated by FHV virions; lane 5, same as lane 4 but without a reverse transcription step. The expected product lengths are indicated on the left of each gel.