Table 1. Morphometric evaluation of jejunal sections.
Treatmentl | Control | Gliadin | B. longum | Gliadin/B. longum | IFN-γ | IFN-γ/Gliadin | IFN-γ/Gliadin/B. longum | |
Villi | width (µm) | 46.15±7.56a | 42.65±6.39 | 57.10±6.23 | 57.53±8.36 | 48.57±6.35 | 38.27±8.46ab | 50.46±2.61ab |
length (µm) | 193.37±15.53c , d | 156.68±29.74 | 255.83±31.57d | 247.44±51.74c | 187.68±26.10 | 165.88±36.62 | 196.74±14.00 | |
Infiltrated cells1 | 7.75±1.25e , f , g | 8.80±1.64 | 8.12±0.80 | 10.24±1.10e | 6.85±2.12h | 11.25±0.96f , h | 10.52±1.57g | |
Enterocytes | height (µm) | 7.04±1.66i , j | 4.29±1.24i , k | 8.32±1.58 | 7.71±1.01k | 7.68±1.92 | 4.79±1.03j , l | 7.22±0.96l |
counts2 | 4.80±0.80m,n | 6.83±0.75 | 5.09±0.62 | 6.75±1.22 | 5.12±097 | 8.20±0.80m | 8.01±0.35n |
The results are expressed as mean ± standard deviation of 20 independent microscopic fields of each animal (n = 6).
Superscript letters in the same row indicate statistically significant (P<0.05) differences between the pair of samples that has the same letter as determined applying the Student t test (*).
Number of cells in a surface of 20 µm2 at the lamina propria;
Number of enterocytes a long 20 µm at the luminal side of the intestinal epithelia.
* P values: a, 0.050; b, 0.004; c, 0.020; d, 0.016; e, 0.004; f, 0.001; g, 0.007; h, 0.043; i, 0.014; j, 0.033; k, 0.005; l, 0.005; m, 0.001; n, 0.001.