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. 2012 Jan 23;83(1):013110. doi: 10.1063/1.3677334

Figure 4.

Figure 4

Feasibility of TIRFM imaging-guided confocal single-molecule fluorescence spectroscopy. (a) Image of Rhodamine 6G molecules in TIRFM mode. (b) The corresponding confocal single-molecule spectroscopy measurement on molecule a in (a). The fluorescence intensity decay from single-molecule photon stamping recording is fitted with exponential decay with fit residual; the fluorescence lifetime of the single Rhodamine 6G molecule is 3.1 ± 0.3 ns. Inset: The intensity trajectory of the single molecule.