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Binding of anti-hIgG labeled with ZnS clusters of different sizes to the surface of microtiter plate well that was completely coated with hIgG. The Zn2+ of ZnS clusters attached to the well was released through acid digestion and quantified by FluoZin-3. This Zn content was then used to calculate the total amount of ZnS clusters, assuming the clusters were solid particles when computed the total Zn in each cluster.
