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. Author manuscript; available in PMC: 2013 Feb 7.
Published in final edited form as: Biochemistry. 2012 Jan 24;51(5):1028–1040. doi: 10.1021/bi201818c

Figure 7.

Figure 7

(A) Potential tunnel for NO diffusion from the heme to the Zn2+-tetrathiolate within iNOS. The tunnel within a structure of the iNOS heme domain (pdb 1DWV) (85) was generated using CAVER (86). (B) Summary model of iNOS auto-inactivation through S-nitrosation of the Zn2+-tetrathiolate and protection from inactivation by reductants. The illustrated scheme is superimposed on the kinetic model that was developed by treating the substrate arginine as a mechanism-based inhibitor. Rescue by cellular reductants (GSH) is also shown as well as trapping of released NO by MbO2.