Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Oct 19;49(2):85–96. doi: 10.1016/j.mcn.2011.10.004

Fig. 2.

Fig. 2

Sh3kbp1 mRNA-3 is expressed in Schwann cells in vitro and in vivo. (A) RT-PCR was performed on cDNA generated from cultured Schwann cells (S16), mouse sciatic nerve (mSN), and cultured motor neurons (MN-1) to test for the presence of Sox10 and Pou3f2 transcripts. (B) Similar RT-PCR assays as described in (A) to detect Sh3kbp1 transcripts harboring exon 1A, exon 1B, and exon 1C. Negative controls without cDNA [‘Blank’ in (A) and (B)] were included for each reaction and primers for β-actin mRNA were used as a positive control. Base pair (b.p.) sizes of markers are provided on the left, and expected band sizes for each product are indicated with an asterisk.