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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Oct 19;49(2):85–96. doi: 10.1016/j.mcn.2011.10.004

Fig. 6.

Fig. 6

SOX10 is sufficient for Sh3kbp1 P3 activity in SOX10-negative MN1 cells. (A) A construct harboring Sh3kbp1 P3 directing luciferase expression was co-transfected into cultured motor neurons (MN-1) with constructs to express POU3F2, SOX10, SOX8, or SOX9. Luciferase assays were employed to test the activity of Sh3kbp1 P3 in the presence of each construct, relative to the activity in the presence of the control (‘Empty’) vector. Error bars indicate standard deviations. (B) cDNA was generated from cultured motor neurons (MN-1) transfected with constructs to express SOX10 or POU3F2, both proteins, or neither (‘Mock’). RT-PCR was then performed to test for the presence of Sh3kbp1 Exon 1C (mRNA-3). Note that the primers used were the same ones employed in Figure 2B. A negative control without cDNA (‘Blank’) was included and primers for β-actin mRNA were used as a positive control. A base pair marker is provided on the far left of the gel.