Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2011 Dec 21;11(2):288–297. doi: 10.1158/1535-7163.MCT-11-0490

Figure 2. FAK and Pyk2 mediate the effects of c-Met on medulloblastoma cell proliferation.

Figure 2

FAK and Pyk2 expressions were silenced in DAOY and D425 cells by transfection with FAK-siRNA (35 nM) or Pyk2-siRNA (35 nM), respectively. Control cells were transfected with 35 nM scrambled siRNA (cont-siRNA). After 24 hours, the cells were treated with 20 ng/ml HGF and counted every day for 4 days. The results show that silencing of FAK or Pyk2 significantly decreases basal and c-Met-induced cell growth. A) D425-FAK silencing, B) D425-Pyk2 silencing, C) DAOY-FAK silencing, D) DAOY-Pyk2 silencing. E) Immunoblots show FAK and Pyk2 knockdown in cells treated with respective siRNA as described above. Results are representative of 3 different experiments.