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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2011 Dec 21;11(2):288–297. doi: 10.1158/1535-7163.MCT-11-0490

Figure 3. FAK and Pyk2 mediate the effects of c-Met on medulloblastoma cell migration and invasion.

Figure 3

FAK and Pyk2 expressions were silenced in DAOY and D425 cells by transfection with FAK-siRNA (35 nM) or Pyk2-siRNA (35 nM), respectively. Control cells were transfected with 35 nM scrambled siRNA (cont-siRNA). After 24 hours, the cells were treated with 20 ng/ml HGF and subsequently, assessed for migration (A) and invasion (B), using the scratch assay and the boyden chamber assay, respectively. The results show that silencing of FAK or Pyk2 significantly decreases basal and c-Met-induced migration and invasion in medulloblastoma cells. The immunoblots in the lower panel show the knock-down of FAK and Pyk2 in DAOY cells treated as described above.