Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Autoimmun. 2011 Dec 7;38(1):1–9. doi: 10.1016/j.jaut.2011.09.004

Figure 1. Reduced RAG/IL-7R expression after anti-IL-6 treatment in DWEYS-MAP immunized BALB/c mice.

Figure 1

Figure 1

BALB/c mice (3-month-old) were immunized with DWEYS-MAP and boosted a week later (see Material and Methods). Anti-IL-6 antibody or isotype control (3–5 mice each group) was administrated to mice intravenously at day 8 and day 14 following the initial immunization. Spleens were collected at day 17. Antigen specific B cells were sorted as shown in (A) and RNA was extracted for qPCR to assess RAG (B) and IL-7R (C) mRNA. Spleen sections were frozen on the same day and immunohistochemistry was performed to detect RAG (left column, staining in green) and DWEYS peptide reactivity (mid column, tetramer staining in blue) levels (D and E).