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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Autoimmun. 2011 Dec 7;38(1):1–9. doi: 10.1016/j.jaut.2011.09.004

Figure 3. Reduced RAG/IL-7R expression in DWEYS-MAP immunized IL-6+/− mice.

Figure 3

IL-6 heterozygous (IL-6+/−) mice (3-month old) and age-matched wild type BALB/c mice were immunized with DWEYS-MAP and boosted a week later (see Material and Methods). Antigen specific B cells were sorted on day 17 following the initial immunization and RNA was extracted for qPCR to check RAG and IL-7R mRNA (A). Spleen sections were frozen on the same day of sorting and immunohistochemistry was performed to detect RAG protein levels (B).