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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Autoimmun. 2011 Dec 7;38(1):1–9. doi: 10.1016/j.jaut.2011.09.004

Figure 4. B cell production of IL-6 is required for re-induction of receptor editing. BALB/c mice (3-month-old) were immunized with DWEYS-MAP and boosted a week later. RNA was extracted from splenocytes at day 17 for qPCR to assess IL-6 mRNA level.

Figure 4

Figure 4

(A). Splenic B cells from 3-month-old IL-6+/− mice or age-matched wild type BALB/c mice were purified and adoptively transferred to μMT mice by intravenous injections. 3 weeks later, the recipient mice were immunized with DWEYS-MAP and boosted a week later (see Material and Methods). Spleens were collected at day 17. Antigen specific B cells were isolated and RNA was extracted for qPCR to assess RAG and IL-7R mRNA (B). Spleen sections were frozen on the same day and immunohistochemistry was performed to detect RAG protein levels (C).