Table 1.
Lyophilisation efficiency—antigens preserved directly after freeze-drying had been completed (day 0 of storage), in materials coming from plants attributed to particular expression groups: low <2 μg/g FW, medium 2–10 μg/g FW and high >10 μg/g FW
| Antigen | Content in initial fresh tissue (μg/g) | Antigen preservation in lyophilised tissue | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Absolute content (μg/g) | Relative content (%) | ||||||||
| Low | Medium | High | Low | Medium | High | Low | Medium | High | |
| M-HBsAg | |||||||||
| S domain | 1.8 | 4.2 | 9.8 | 24.5 | 26.1 | 34.2 | 94.3 | 43.6 | 24.5 |
| preS2 domain | 1.3 | 3.8 | 9.0 | 7.2 | 8.1 | 9.1 | 40.1 | 14.8 | 7.1 |
| VLPs | 0.4 | 0.8 | 1.1 | 0.8 | 5.5 | 14.6 | 13.2 | 48.2 | 93.8 |
| L-HBsAg | |||||||||
| S domain | 1.0 | 3.1 | 5.7 | 13.3 | 16.2 | 15.9 | 94.2 | 37.2 | 19.6 |
| preS1 domain | 0.6 | 2.8 | 4.1 | 7.9 | 8.0 | 7.7 | 91.8 | 19.8 | 13.1 |
| VLPs | 0.3 | 0.5 | 0.2 | 3.8 | 2.3 | 1.2 | 95.9 | 33.9 | 38.2 |
Antigen content in fresh tissue is a mean from 6 to 8 plants of T1 generation used for lyophilisation; the antigen content in T1 generation decreased in comparison to T0 generation, when expression groups were distinguished
Relative content calculated as a ratio of obtained value and theoretical value, if whole antigen would be preserved and assuming that dry mass accounts for 7% of leaf fresh weight; dry mass determined using the gravimetric method (May et al. 1989)